![]() ![]() The role of genomic epidemiology is important in the successful control and prevention of the COVID-19 pandemic. The laboratory has now extended its offerings to facilitate molecular and genomic diagnosis that will provide insights into rare disease treatments. Set up in only 14 days to cater to the urgent scale-up for COVID-19 testing in early 2020, Biogenix Labs have played a crucial role in the 4Humanity Phase III inactivated vaccine trials and is a frontrunner in diagnostics and viral genome sequencing in the UAE and region. In the long term, Oxford Nanopore sequencing unlocks many possibilities for the analysis of other pathogens, microbial surveillance, and larger public health impact that we look forward to exploring.” He added: “The ARTIC Midnight Kit will allow us to deliver high-quality SARS-CoV-2 genomic sequencing faster, with more flexible workflows and cost optimization. As the UAE’s first COVID-19 accredited large-scale throughput testing laboratory, Biogenix Labs upholds the safety of communities and we have so far carried out more than 2.5 million RT PCR and saliva tests that have enabled safe return to work, seamless travel and helped children go back to school.” Nanopore sequencing and ARTIC Midnight Kit can achieve higher coverage with a lower number of reads, producing less ambiguous base calls, and is affordable when compared to alternative approaches (reference).ĭr Fahed Al Marzooqi, Chief Operations Officer, G42 Healthcare, said: “We are delighted with this certification and the opportunity to enhance rapid insights that can be swiftly actionable for public health policy. The barcoding capacity which ensures thousands of samples in a single sequencing run per instrument coupled with a rapid analysis pipeline post sequencing, makes the setup key in a pandemic situation, allowing scientists and healthcare workers to have up to date variant information for disease management. The ARTIC Midnight protocol uses overlapping PCR amplified targets across the viral genome, giving the advantage of whole genome surveillance rather than focusing only on the gene that codes for the viral spike protein. This capacity and volume of samples sequenced at Biogenix Labs positions it as a preferred referral lab for COVID-19 epidemiology through genome sequencing. The protocol is highly scalable and can be deployed on Oxford Nanopore’s range of sequencers such as the MinION, GridION and PromethION depending on the sample volumes and turnaround time. With this, Biogenix Labs becomes a Certified Service Partner for Oxford Nanopore and the first lab in Middle East to be certified for providing cost-effective, high accuracy sequencing for genomic epidemiology for COVID-19 strain surveillance in RT-PCR positive cases with a quick turnaround time. SARS-CoV-2 Midnight Amplicon Panel and xGen DNA Library Prep Kit EZ metrics.( MENAFN- APCO Worldwide) Abu Dhabi, January 23rd, 2022: Biogenix Labs, the UAE’s first COVID-19-accredited large-scale testing laboratory and part of Abu Dhabi-based leading health tech company, G42 Healthcare, has received certification for the use of Oxford Nanopore sequencing technology to conduct SARS-CoV-2 sequencing for COVID-19 surveillance and variant detection. When the SARS-CoV-2 Midnight Amplicon Panel and xGen DNA Library Prep Kit EZ are combined, the recommended Ct value is <32 which has been shown to sequence 97% of the SARS-CoV-2 genome with sufficient depth to identify variants (Table 1). In our experiment, contrived samples were prepared by mixing inactivated SARS-CoV-2 viral material into a negative background matrix of a negative nasopharyngeal (NP) swab in viral transport medium (VTM), with two samples per Ct value. Samples with less viral genomic material pose difficulty for primer annealing The C t value for the amplicon sequencing reaction is an important metric since the higher the C t, the lower the number of viral genomes in the sample. This results in a lowered chance of amplicon dropouts. Since the primers only anneal to a total of 4.5% of the viral genome, the chance that viral mutations will disrupt primer annealing is lower. Target Capture Probe Design & Ordering Tool. ![]() Library Concentration Conversion Calculator. ![]()
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